Precautions And Safety Measures To Consider While Using ELISA Kits
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When buying an ELISA kit, it often comes with directions from the Elisa kits suppliers in India. The reason is that there are several precautions that you must consider while using these kits. Today, we are going to talk about some of the safety measures that will ensure protection.
Precautions While Using ELISA Kits
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This kit is ideal for research purposes only and not for diagnosing or therapeutic uses.
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One must quit using the ELISA kit after its expiration date.
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Try not to mix reagents into the kit belonging to distinctive lots.
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The kit is made and tested for detecting specific samples as well as targets present in the manual. Further use of it for any other purpose must be done very carefully by the end-users.
Safety Instructions On An ELISA Kit
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The stop solution that you get with the ELISA kit is generally an acid solution. Make sure to use the reagent with proper safety for avoiding any kind of risk.
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You must handle as well as discard all biological materials because they are potentially harmful materials. Hence, make sure to follow local rules and regulations.
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Personal security equipment such as gloves, lab coats, goggles and surgical masks are a must for these experiments. Inhaling any of these substances can have side effects.
Tips To Make Any ELISA Assay Successful
No ELISA assay can become successful overnight. End numbers of efforts can make that happen. Given below are some useful tips for a victorious ELISA assay.
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Consistency is the key
While consistency may appear like an obvious goal, there are a few assumptions that may affect its presence between plates. One should resist mixing different components in ELISA kits because they can result in potential damage.
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Ensure accuracy in the assay
You can strive to increase its accuracy by ensuring that you have a duplicate at each point in the assay. This may include anything from the background to the sample wells. Most significantly, it will allow you to identify any outlier immediately.
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Prevent contamination
The key to a successful ELISA assay is having a low coefficient of variability in your samples. Make sure none of them exceeds their ideal percentage because it can lead to further contamination.
Conclusion
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