The Q800R Sonicator may be an effective chromatin and DNA shearing framework, perfect for analysts looking for cost-effective, high-quality DNA fracture. Competent to create DNA parts between 150bp and 3kb, the framework permits for preparing up to 18 tests at the same time. The Q800R Sonicator is congruous with different test tubes and suits volumes from 50µl to 1ml. With its coordinate chiller, advanced working framework with programmable memory, and sound-reducing walled in area, this framework gives improved ease of use, calmer operation, and prevalent execution. As a driving Q800R3 Sonicator Supplier in India Biochain guarantees dependability comes about and fetches effectiveness for all DNA shearing needs.
Features-
High Throughput:
The Q800R3 framework is planned for high-throughput DNA shearing, permitting the preparation of up to 18 tests at the same time. This highlight is especially useful for research facilities that require large-scale test preparing, moving forward efficiency and minimizing preparing time. Analysts can effectively shear DNA over a wide extent of part sizes (150bp to 3kb) without compromising on execution.
Employments Standard Test Tubes:
The framework is congruous with commercially accessible test tubes, making it simple to coordinate into existing workflows without the requirement for specialized consumables. This flexibility permits clients to work with standard 50µl to 1ml test volumes, employing an assortment of broadly accessible tubes, decreasing costs and guaranteeing comfort for scheduled applications.
Two-Year Guarantee:
The Q800R3 comes with a comprehensive two-year guarantee, giving clients peace of intellect with respect to the system's unwavering quality and execution. This guarantee guarantees that any potential issues are tended to instantly, minimizing downtime and maximizing the system's life span, making it a shrewd, risk-free speculation for analysts.
PART NO. Q800R3 INCLUDES:
- Generator
- Converter
- Ultrasonic Horn
- Rotation System
- Chiller
- Cables
- Tubing
- 3 Tube Rack Options
- 2-Year Warranty
Chromatin Shearing - Sample Protocols
| Sample Type | Mammalian Chromatin | Yeast Chromatin | C. elegans Chromatin |
|---|---|---|---|
| Cell Type | HEK 293T (2) 10cm dishes 70-80% confluent | Wild type S. pombe cells grown to an OD of 1.3-1.5 in YEA | C. elegans culture; Nuclear extract 1mg/ml |
| Total sample volume | 300ul | 300ul | 500ul |
| Fixation time | 1% Formaldehyde, 13 min | 1% Formaldehyde, 15 min | 2% Formaldehyde, 20 min |
| Sonicator Amplitude Setting | 70% | 100% | 70% |
| Sonication Pulse Rate | 15 seconds On, 45 seconds Off | 20 seconds On, 40 seconds Off | 30 seconds On, 30 seconds Off |
| Total Sonication On Time | 30 min | 30 min | 20min |
DNA Shearing - Sample Protocols
| Sample Type | Bacterial Genomic DNA | Human Skeletal Myoblast Genomic DNA |
|---|---|---|
| Cell Type / Concentration | E. coli / 250 ng | LHCN-M2 Skeletal Myoblast / 200ng |
| Sonication Pulse Rate | 15 seconds On, 15 seconds Off | 15 seconds On, 15 seconds Off |
| Total Sample Volume | 200ul in 0.5ml tubes | 200ul in 0.5ml tubes |
| Sonicator Amplitude Setting | 70% | 100% |
| Total Sonication On Time | As indicated below | As indicated below |
| Sonicator Amplitude Setting | 20% | 20% |